The conference to elect the successor has been delayed many times, this time due to the outbreak of Covid-19. The position of deputy chair is also vacant after Violet Siwela became an MP.
Provincial police spokesperson Brig Leonard Hlathi said the five are facing charges of murder, intimidation, public violence and assault with intent to cause grievous bodily harm (GBH).
Hlathi said they are expecting more arrests on the case.
"We can confirm that one person is on the run and we expect more (people) to be arrested as the investigation continues," said Hlathi.
Also in March, another man lost his life during an ANC meeting at ward 17, at Msukaligwa local municipality in Ermelo, in what is believed to be a leadership dispute between party members.
Sowetan can reveal that the Lukheleni siblings are sons of a well-known taxi boss.
A member who attended the KaBokweni meeting told Sowetan that only Mabuza and Ngomane belong to their branch and said they believed the Lukheleni brothers were "hired to disrupt the meeting".
"Everybody knows them here; they stay in the same township but not in ward 32. We got information that they were called to 'solve' people who disagree with Mabuza and Ngomane in the meeting," said the woman.
Another independent source from the ANC corroborated the information.
"What we know is that Manzini was targeted by these people but not only him, even ourselves if we happened to be in that meeting, we were going to be assaulted or maybe shot," said the source.
A week before Manzini was killed, another meeting was called off after a shot was fired. At the time, Ndlovu condemned the incident.
Manzini's brother, Joe Manzini, said their wish was for the accused to reveal the names of those behind the disruption that created the murder of his brother.
"We are happy that there are people arrested regarding my brother's death.
"We wish that they don't get bail before they tell us who was behind the killing because it's clear they were bought to kill my brother.
"The court is our hope now, we're asking for justice for our brother," said Joe.
Spokesperson for the ANC in the province, Sasekani Manzini (not related to the deceased), commended the police for the arrest of the five.
"As the ANC, we want to see anyone who has been found to have broken the law to be prosecuted. We are therefore happy that the law is taking its course on this matter involving the death of a member of the ANC.
"Even if people are members of the ANC, they have no right to kill anyone, hence we are saying the law must take its course," Sasekani Manzini said.
She said the party is still going to check on how the Lukheleni brothers found themselves in a meeting outside their ward.
"The ANC is going to find out if these three people from ward 33 are real members of the ANC and how did they happened to be in a meeting of the ANC in another ward."