Oscar "Blade Runner" Pistorius will race against Olympic 400m champion Jeremy Wariner in July.
He will run in his first able-bodied Grand Prix event at the Norwich Union Sheffield Grand Prix on July 15.
"With the likes of Jeremy Wariner in the field I couldn't ask for a stronger test, but these are the sort of challenges I want," said Pistorius, 20.
He runs on carbon fibre curved blades after his legs were amputated below the knee when he was 11 months old.
The South African was born without fibulae in both legs because of a congenital condition. Other big names in the 400m at the Sheffield meet include Britain's No 1 Tim Benjamin and Commonwealth champion John Steffenson from Australia.
In March, it looked as though Pistorius would not get the chance to compete against able-bodied runners after the IAAF, athletics' governing body, introduced a rule banning any runner deemed to benefit from artificial help.
However, the IAAF subsequently said Pistorius could compete and wants to use the Sheffield event as part of its research into whether or not he gains an advantage from his blades.
Former Olympic 400m silver medallist Mark Richardson told BBC Radio Sheffield he believes Pistorius should be allowed to race against able-bodied athletes.
"There should be no reason why he shouldn't." - BBC