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Officials expect 3.5 million people to converge on the 2.5-mile (4-km) parade route in Manhattan for the annual, nationally televised ritual that initiates the holiday shopping season with giant balloons shaped like cartoon characters.
The 82 sand trucks from the Sanitation Department will be used to counter a threat by Islamic State, also known as ISIS, ISIL or Daesh, which has called the parade an "excellent target."
The group has encouraged readers of its online magazine Rumiyah to use motor vehicles to kill and injure people, similar to the way a Tunisian-born assailant killed more than 80 people at a Bastille Day celebration in Nice, France.
The sand trucks weigh 16 tons (14.5 metric tonnes) and twice that when filled with sand, making them difficult to move even if rammed by large vehicles.
Islamic State, which controls swathes of Iraq and Syria and seeks to inspire attacks by others abroad, claimed responsibility for the July 14 attack in Nice.
"We are aware of some of the reports that have been out there, but I want to assure all New Yorkers there's no credible and specific information of any specific threat directed toward this parade," New York Mayor Bill de Blasio told a news conference on Wednesday.
Police have also used the sand trucks to protect nearby Trump Tower, the home and campaign headquarters of President-elect Donald Trump.
The parade, due to begin at 9 a.m. EST (1400 GMT), will start in Central Park and run down Sixth Avenue, one block from Fifth Avenue where Trump Tower is located, and end near the Macy's department store on 34th Street.
De Blasio estimated 250,000 people came out on Wednesday to watch the giant balloons being inflated.
"The NYPD is ready," de Blasio said. Police have been preparing "literally since the end of last year's parade," he said.
This year police will ban vehicle traffic from crossing the parade route, unlike in past years when a few cross streets were open, requiring the trucks and 114 smaller blocker cars.
"We get paid to keep people safe and we'll keep people safe tomorrow," Police Commissioner James O'Neill said.