Model, actress and radio personality Pearl Thusi was in a car accident that left her and her daughte.
Botha was facing seven charges of attempted murder and was then removed from the Pistorius case. Senior investigator, LT General Vinesh Moonoo had since then replaced Botha.
He and two other officers were arrested a year ago after shooting at a minibus taxi carrying passengers.
Police spokesperson Neville Malila said, “We can confirm that he tendered in his resignation yesterday [Wednesday] but the reasons are confidential.”