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The 'Anaconda' star's team practice didn't go to plan when a female dancer was attacked by a six-foot serpent.
According to gossip website TMZ, the unfortunate performer was bitten by the reptile and had to be taken to hospital, where she is being treated.
It is believed an anaconda was used - in a direct reference to Nicki's new track - and while it is a non-venomous snake, they can often transmit bacteria that could lead to serious infections.
The serpent was removed from the stage in a glass container, and it's not yet known if it will be used in the live performance this weekend.
As well as her performance of 'Anaconda', Nickie was also join Jessie J and Ariana Grande to open the show with a their collaboration 'Bang Bang'. Meanwhile, she also be joined Usher for a rendition of 'She Came To Give It To You'.